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Fisher Scientific tryple express
Tryple Express, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tryple+express/express+tryple/pmc13065922-530-21-23
Average 86 stars, based on 1 article reviews
tryple express - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

Fluorescence:

Article Title: Generation of a homozygous and heterozygous iPSC line carrying a variant of uncertain significance in CACNA1C, associated with Brugada syndrome.
Article Snippet: Monthly mycoplasma screening (passage 25–35) with the LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich). .. HiPSCs were singularized with TrypLE Express (Fisher Scientific; 5 min, 37 ◦C), resuspended in P/S-free SF medium, and counted on a LUNA automated fluorescence cell counter (Westburg). .. For each nucleofection, 1 × 106 cells were transfected using a 4D-Nucleofector (Lonza) with the V4XP-3032 kit (Lonza) and program DS-138.

Cell Culture:

Article Title: Krisanaklan Reduces Intestinal Anion and Fluid Secretion Through Inhibition of Na + /K + -ATPase and K + Channel Activity.
Article Snippet: For intestinal epithelial current measurements, cells were seeded (1×105 cells/cm2) on a permeable membrane support (Transwell #3470; Corning) that had been pretreated with collagen type I (10 μg/mL in phosphate buffered saline, 0.3 mL/cm2, 2 h, 37◦C), and cultured for ~14 (HT29-CL19A, T84) or 30 (Caco-2) days. .. Cell culture media and TrypLE Express were obtained from Gibco/Thermo Fisher Scientific. .. Further chemicals, including analytical grade inorganic salts, were purchased from SigmaAldrich, except BPO-27 (MedChemExpress.com), KK (Kilin brand, Thailand), linaclotide (Adooq Bioscience), nystatin (Santa Cruz Biotechnology), and vasoactive intestinal peptide (VIP; Bachem).

Article Title: NR2F6 deletion revives CAR-T cell function and induces antigen-agnostic immune memory in solid tumors
Article Snippet: .. Briefly, cell culture medium was collected in a 15 mL Falcon tube, wells were wash with 1x PBS and 400 μL TrypLE Express (Fisher Scientific, 12604013) was added per well. ..

Article Title: NR2F6 deletion revives CAR-T cell function and induces antigen-agnostic immune memory in solid tumors.
Article Snippet: .. Briefly, cell culture medium was collected in a 15mL Falcon tube, wells were wash with 1x PBS and 400μL TrypLE Express (Fisher Scientific, 12604013) was added per well. ..

Incubation:

Article Title: Generation of apical-out nasal organoids to facilitate viral infection and drug screening
Article Snippet: .. After an additional spin and removal of the supernatant, they were resuspended in TrypLE Express (Fisher Scientific, cat # 11558856) and incubated for 5-8 min at RT, before being dissociated to single cells by pipetting vigorously with a P1000 pipette. .. The single-cell suspension was diluted 1:1 with Trypan Blue (STEMCELL Technologies, cat # 07050), loaded to a hemocytometer and imaged in Leica DMi8, where the ciliated cells were counted manually.

Transferring:

Article Title: Generation of apical-out nasal organoids to facilitate viral infection and drug screening
Article Snippet: .. After an additional spin and removal of the supernatant, they were resuspended in TrypLE Express (Fisher Scientific, cat # 11558856) and incubated for 5-8 min at RT, before being dissociated to single cells by pipetting vigorously with a P1000 pipette. .. The single-cell suspension was diluted 1:1 with Trypan Blue (STEMCELL Technologies, cat # 07050), loaded to a hemocytometer and imaged in Leica DMi8, where the ciliated cells were counted manually.



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